Composite

Part:BBa_K4902008

Designed by: Liam Davidsson   Group: iGEM23_Chalmers-Gothenburg   (2023-10-10)


FMN -> alpha-ribazole

The construct is made up of two operons which together express the genes needed to complete the pathway between riboflavin-5′-phosphate and alpha-ribazole and its eventual conjugation into cobalamin (vitamin B12). The genes riboflavin reductase (two subunits) and dimethylbenzimidazole synthase share one of the operons, controlled by the erythromycin promotor and tailed by a double T7Te + rrnB T1 terminator. The second operon is under the control of the CP44 promotor and the same T7Te + rrnB T1 double terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal BamHI site found at 2982
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 912
    Illegal EcoRI site found at 3117
    Illegal SpeI site found at 4060
    Illegal AgeI site found at 2379
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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